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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Fibroblast-derived CXCL12 regulates PTEN expression and is associated with the proliferation and invasion of colon cancer cells via PI3k/Akt signaling

Fig. 2

Effect of recombinant CXCL12 and co-culture with fibroblasts on PTEN Relative expression of PTEN mRNA in colon cancer cell lines. The alteration of PTEN mRNA from colon cancer cell lines[HT-29 (a), Colo320 (b), and CaCo-2 (c)] by recombinant CXCL12 stimulation, co-culture with fibroblasts (FB) or co-culture with fibroblasts+anti CXCL12 antibody were determined by semi-quantitative RT-PCR. The experimental detail is described in the “Materials and Methods” section. Control: colon cancer cells only; FB:co-culture with fibroblasts; CXCL12: treated with recombinant CXCL12; FB + Ab: colon cancer cells co-cultured with fibroblasts and pre-treated with anti-CXCL12 Ab. The values are expressed as mean ± SD. Multiple comparisons were performed by one-way ANOVA followed by Dunnett test. Bars indicate SD

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