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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Fibroblast-derived CXCL12 regulates PTEN expression and is associated with the proliferation and invasion of colon cancer cells via PI3k/Akt signaling

Fig. 1

Expression levels of PTEN, CXCL12 and CXCR4 in colon cancer cell lines and stromal cells. (a) PTEN, CXCL12 and its receptor CXCR4 mRNA were detected by RT-PCR in colon cancer cells and fibroblasts. PCR-amplified products of reverse-transcribed mRNA (cDNA) from GenBank, using primers specific for PTEN, CXCL12 and CXCR4 PCR products, were separated through 2% agarose gels and stained with ethidium bromide. β-actin served as a loading control. (b) The protein expression levels of CXCL12 and CXCR4 in colon cancer cell lines and fibroblasts were determined in whole-cell lysates by Western blotting analysis. Thirty micrograms of total cell lysate was subjected to 10% SDS-PAGE and transferred to polyvinylidene difluoride membrane. The membrane was probed with antibodies to PTEN, CXCL12 and CXCR4. β-actin acted as a loading control

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