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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: 4.1B suppresses cancer cell proliferation by binding to EGFR P13 region of intracellular juxtamembrane segment

Fig. 5

Hyperproliferation and enhanced EGFR-mediated signal transduction of immortalized 4.1B KO MEF cells. a Proliferation of immortalized WT MEF and 4.1B KO MEF. b Increased phosphorylation of EGFR, ERK and AKT in 4.1B KO MEF cells. β-actin served as the loading control. c Effects of erlotinib on cell proliferation. d Expression of EGFR mRNA levels in various cell types as indicated: GFP or GFP-4.1B -overexpressing MGC-803 cell lines (a); MKN-45 or 4.1B KO MKN-45 cells (b); immortalized WT MEF and 4.1B KO MEF (c). e Decreased expression of Sp1 mRNA and protein levels in 4.1B overexpressing MGC-803 cells (a; b) and increased expression of Sp1 mRNA and protein levels in 4.1B-deficient MKN-45 cells (c; d) and 4.1B KO-MEF cells (e; f). f Decreased expression of EGFR in Sp1 knockdown cells (4.1B-deficient MKN-45 cells mRNA level (a) and protein level (b)); (4.1B KO-MEF cells mRNA level (c) and protein level (d)). * p < 0.05, ** p < 0.01, *** p < 0.001

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