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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Phloretin attenuates STAT-3 activity and overcomes sorafenib resistance targeting SHP-1–mediated inhibition of STAT3 and Akt/VEGFR2 pathway in hepatocellular carcinoma

Fig. 2

PH inhibits STAT3, AKT/mTOR and RAS/MAPK signaling in HCCs. a Cells were treated for 48h with indicated concentrations of PH and phosphorylated and total protein levels of the target proteins were evaluated by Western blotting. b pSTAT-3 activity was measured by ELISA (c) PH inhibits cytoplasmic, nuclear STAT3 phosphorylation in HCCs at 50uM. d Cells (wild-type or ectopic expression of STAT3) were exposed to PH at 50uM for 24 hours. Percentage of apoptotic cells was analyzed by flow cytometry. e Representative images from two patients with double immunostaing of CD31/SHP1 and pSTAT3. Experiments were conducted in triplicate and mean values ± SD (bars) are shown. *p<0.05, and **p<0.01 compared to control

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