Fig. 4
From: Nonlytic exocytosis of Cryptococcus neoformans from neutrophils in the brain vasculature
Neutrophils expel C. neoformans in the brain vasculature. Neutrophils were stained with anti-Ly6G (pseudocolored red); C. neoformans was stained with the anti-capsule antibody E1 (pseudocolored green), and the brain vasculature was stained with anti-collagen IV (pseudocolored blue). a Neutrophils containing C. neoformans in the brain vasculature; original scale bar, 20 μm; b Two fungal cells located in a neutrophil attached to a brain capillary. One fungal cell seemed to be expelled from the neutrophil; original scale bar, 10 μm. c A 3D reconstructed image based on z-stack images was captured for further analysis