Fig. 2
From: Nonlytic exocytosis of Cryptococcus neoformans from neutrophils in the brain vasculature
Flow cytometry analysis of nonlytic exocytosis. a After incubation, live neutrophils with intracellular C. neoformans (FITC+DDAO-SE+ 7-AAD−Unvitex2B−) were sorted. b Immediately after sorting, the cells were analyzed for purity. c After 2 h of incubation with or without drugs, neutrophils with C. neoformans were collected, stained with 7-AAD and further analyzed. FITC−DDAO-SE+ 7-AAD− cells were considered neutrophils undergoing nonlytic exocytosis. Representative results of 5 repeated experiments. d Fungal capsule and viability regulate nonlytic exocytosis. Sorted live neutrophils containing either wild-type C. neoformans (B3501, H99) or acapsular strains (Cap67, Cap59) were incubated for 2 h. Nonlytic exocytosis was calculated as described by André Moraes Nicola, mBio, 2011. Heat-killed H99 cells were included to explore whether viability affects nonlytic exocytosis. *, p < 0.05