Fig. 8

Differential regulation of InsP1 accumulation and Ca²⁺-release induced by EB. InsP1 accumulation was measured due to InsP3 production. a, b Human washed platelets (WP) were pre-incubated as described before with the various reagents used in the previous experiments prior to stimulation with EB in presence of 1 mM LiCl. Aggregation was stopped after 5 min using the lysis buffer provided by the manufacturer. c WP preincubated with Fluo-3, AM (5 μM) for 30 min at 37 °C before treating platelets. Ca²⁺-release was monitored for 120 s using flow cytometry. Data are representative of 3 different experiments from 3 healthy donors. Results are means ± S.D. ns: not significant, *p < 0.1, ***p < 0.001, ****p < 0.0001