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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: cAMP- and cGMP-elevating agents inhibit GPIbα-mediated aggregation but not GPIbα-stimulated Syk activation in human platelets

Fig. 7

Iloprost and riociguat inhibit EB-induced platelet aggregation but do not inhibit tyrosine phosphorylation of Syk. Washed platelets (WP) were pre-incubated for 3 or 2 min with 2 nM iloprost or 20 μM riociguat respectively prior to stimulation with EB. a Aggregation curves and the corresponding quantitative data are shown in b. c Aggregation was stopped after 1, 2 or 5 min using Laemmli buffer. Syk Y525/526 and Y352, Syk downstream effectors LAT Y191, PLCγ2 Y759 and Akt S473 were analyzed by western blot. d, e, f, g, h Quantification of the phosphorylated proteins are represented as ratio compared to the total protein or compared to β-actin for LAT analysis. Results are presented of least 3 different experiments with platelets from at least 3 healthy volunteers, data are represented as means ± S.D. ns: not significant, *p < 0.1, **p < 0.01, ***p < 0.001, ****p < 0.0001

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