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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: cAMP- and cGMP-elevating agents inhibit GPIbα-mediated aggregation but not GPIbα-stimulated Syk activation in human platelets

Fig. 5

ADP and TxA2 regulate EB-induced platelet aggregation and Syk downstream signaling. Human washed platelets (WP) were pre-incubated for 5 min simultaneously with P2Y12 antagonist, AR-C69931 (0.1 μM), P2Y1 antagonist, MRS2179 (1 μM) and TxA2 receptor antagonist, SQ-29548 (1 μM) prior to stimulation with EB. Samples were taken after 1, 2 and 5 min. a Representative curves of platelet aggregation mediated by EB in the presence of the vehicle control or the 3 inhibitors and the corresponding quantification are represented in b. c Representative blots of Syk phosphorylation on Y525/526 and Y352 and the corresponding quantification shown as ratio compared to the total Syk protein represented in d and e respectively. f Representative blots of Syk downstream effectors PLCγ2 Y759 and Akt S473 and the corresponding quantification g and h shown as ratio compared to the total PLCγ2 and Akt, respectively. Data are presented as means ± S.D. from 3 independent experiments with 3 healthy donors; ns: not significant, *p < 0.1, **p < 0.01, ***p < 0.001, ****p < 0.0001

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