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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: cAMP- and cGMP-elevating agents inhibit GPIbα-mediated aggregation but not GPIbα-stimulated Syk activation in human platelets

Fig. 4

Syk inhibitors differentially affect Syk tyrosine phosphorylation. Human washed platelets (WP) were treated as mentioned in Fig. 3. Samples were taken after 15, 60 and 120 s. a Representative blots of Syk phosphorylation on Y525/526 and Y352 and the corresponding quantification shown as ratio compared to the total Syk protein represented in b and c respectively. d Representative blots of Syk downstream effectors PLCγ2 Y759 and Akt S473 and the corresponding quantification e and f shown as ratio compared to the total PLCγ2 and Akt respectively. Data are means ± S.D. from 3 independent experiments with platelets from 3 healthy donors; ns: not significant, ***p < 0.001, ****p < 0.0001

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