Fig. 4From: cAMP- and cGMP-elevating agents inhibit GPIbα-mediated aggregation but not GPIbα-stimulated Syk activation in human plateletsSyk inhibitors differentially affect Syk tyrosine phosphorylation. Human washed platelets (WP) were treated as mentioned in Fig. 3. Samples were taken after 15, 60 and 120 s. a Representative blots of Syk phosphorylation on Y525/526 and Y352 and the corresponding quantification shown as ratio compared to the total Syk protein represented in b and c respectively. d Representative blots of Syk downstream effectors PLCγ2 Y759 and Akt S473 and the corresponding quantification e and f shown as ratio compared to the total PLCγ2 and Akt respectively. Data are means ± S.D. from 3 independent experiments with platelets from 3 healthy donors; ns: not significant, ***p < 0.001, ****p < 0.0001Back to article page