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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: N822K- or V560G-mutated KIT activation preferentially occurs in lipid rafts of the Golgi apparatus in leukemia cells

Fig. 6

Lipid rafts have a role in KIT signaling at the Golgi apparatus. a-c Kasumi-1 or HMC-1.1 cells were treated with 0~40 μM cer-C6 for 8 h (for inhibition of normal lipid raft formation). a Lysates were immunoblotted. b Cells treated with 40 μM cer-C6 for 8 h were immunostained for KIT (green), giantin (Golgi marker, red), or GM130 (Golgi marker, blue). Bars, 10 μm. c Pearson’s R correlation coefficients were calculated by analyzing the intensity of KIT vs. giantin (Kasumi-1) or GM130 (HMC-1.1). Results are means ± s.d. (n = 16~22). **P < 0.01. d & e GIST-T1 cells were treated with 0~10 μM cer-C6 for 10 h. d Lysates were immunoblotted. e Lysates were treated with PNGase F or endoglycosidase H then immunoblotted with anti-KIT. CG, complex-glycosylated form; HM, high mannose form; DG, deglycosylated form. f HMC-1.2 cells were treated with 0~40 μM cer-C6 for 8 h, then immunoblotted

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