Fig. 1

N822K- or V560G-mutated KIT mis-localizes to vesicular structures in leukemia cells. a Schematic representations of wild-type KIT (KIT^WT) and constitutively active KIT mutants (KIT^N822K and KIT^V560G) showing the extracellular domain (ECD), the transmembrane domain (TM), the kinase domain, the lysine mutation at 822 (K in red), and the glycine mutation at 560 (V560G). b Kasumi-1, SKNO-1, HMC-1.1, or pt18 cells were immunostained with anti-KIT. Bars, 10 μm. Note that KIT^WT localized to the PM, whereas KIT mutants accumulated on vesicular compartments