Fig. 3
AO treatment specifically inhibits the proliferation of PDAC cells. a The IC50 values of AO for indicated cell lines. Cells were treated with AO at various concentrations for 48 h and processed for the MTT assay. b-d SW1990, AsPC-1 and PANC-1 cells were separately transfected with the scrambled siRNA (control) and GOT1-specific siRNA for 48 h, and the knockdown efficiency of GOT1 levels was analyzed by Western blotting. Then the cytotoxicity of AO on the GOT1 knockdown cell lines were markedly decreased compared with corresponding control cell lines. e-g SW1990, AsPC-1, PANC-1 cells were pretreated with OAA, Asp at indicated concentrations for 2 h, and then incubated with indicated concentrations of AO for 5 days. The cell number was determined by MTT assay. h A CETSA was performed to evaluate the interaction between AO and GOT1 a in SW1990 intact cells and cell lysates. i DARTS assay was performed on the SW1990 cell lysates