Fig. 3

The loss of parkin decreases ethanol-induced lipid accumulation and regulates expression of genes involved in lipid metabolism in mouse hepatic cells. a Immunoblots of parkin and Oil red O staining for lipid accumulation in primary hepatic cells from WT or parkin KO mice treated with ethanol (100 mM) or without. b Expression of genes involved in lipid metabolism in primary hepatic cells from WT or parkin KO mice treated with ethanol (100 mM) or without ethanol (Scale bars, 50 μm). Values are expressed as the mean ± SD of three different experiments conducted in triplicate. ^*P < 0.05, WT versus WT treated with ethanol; ^**P < 0.05, WT versus KO; ^#P < 0.05, WT treated with ethanol versus KO treated with ethanol