Fig. 5

Exosomal CLEC3B decreased VEGF via activating phosphorylation of AMPK in HCC cells. a KEGG enrichment of CLEC3B-correlated genes in TCGA-LIHC database. Points with circled marks were related to Environmental Information Processing, and red related to Signaling transduction while green related to Signaling molecules and interaction. b Relative mRNA expression of VEGF in tumor cells with 3B (no CC, P = 0.0022; CC, P = 0.2697; among groups, P < 0.0001) or 3B-KD (no CC, P = 0.0372; CC, P = 0.1357; among groups, P = 0.0001) and tumor cells treated with Exo-3B (CC, P = 0.0005; no CC, P = 0.0016; among groups, P < 0.0001) or Exo-3B-KD (CC, P = 0.009; no CC, P = 0.0784; among groups, P < 0.0001). c Protein levels in HCC cells with 3B or 3B-KD and treated with Exo-3B or Exo-3B-KD, Compound C (CC) or not. d Secretion of VEGF in supernatants of tumor cells with 3B (no CC, P = 0.0101; CC, P = 0.3572; among groups, P = 0.0004) or 3B-KD (no CC, P = 0.1832; CC, P = 0.4912; among groups, P < 0.0001) and treated with Exo-3B (no CC, P = 0.0123; CC, P = 0.1702; among groups, P < 0.0001) or Exo-3B-KD (no CC, P = 0.0340; CC, P = 0.5750; among groups, P = 0.0004). e Relative tube formation of ECs treated with supernatant from recipient Huh7 cells treated with Exo-3B (no CC, P = 0.0265; CC, P = 0.9874; among groups, P < 0.0001) or Exo-3B-KD (no CC, P = 0.0281; CC, P = 0.6746; among groups, P = 0.0045). *, P < 0.05; **, P < 0.01; ***, P < 0.001; n.s., not significant