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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction

Fig. 1

ITM2A was down-regulated and low ITM2A showed poor clinical outcomes in human breast cancer.(a) Distribution of fold changes illustrated in gene expression profiles for the breast cancer tissues obtained from the TCGA database. The log2 values were calculated for each sample by normalizing to read counts alone (log2 Fold Change). Gene expression analysis was performed using GeneSpringGX software. (b) The mRNA level of ITM2A in nonpaired (left, GSE86374) and paired (right, GSE109169) breast cancer tissues from the GEO database. (c and d) The mRNA and protein levels of ITM2A in the human breast cancer cell lines MCF-7, MDA-MB-231, MDA-MB-468, BT474, and SKBR-3 and the normal breast epithelial cell line Hs578Bst, as well as the human embryonic kidney cell line HEK293T. (e) Representative immunohistochemical staining images of ITM2A expression in human breast cancer tissues (I, II and III) and para-carcinoma tissues. (f) The immunohistochemical staining intensity of ITM2A expression in breast cancer tissues and para-carcinoma tissues. The intensity of the ITM2A immunoreaction was scored as 0, none; 1, weak; 2, moderate; and 3, intense. (g-j) Kaplan-Meier survival curves for the association between ITM2A expression and the probability of overall survival of human breast cancer patients. “Patients at risk” refers to patients who are at risk of the event occurrence, such as death or metastatic relapse. (k) The ROC curve of ITM2A expression in human breast cancer patients. Analysis of the ITM2A expression levels resulted in an area under the curve (AUC) value of 0.935. Data are presented as the mean ± SD. Two-tailed Student’s t-test was used. *P <  0.05; **P <  0.01; ***P <  0.001

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