Fig. 3

Blockade of lactate transport increases GlaB effect. a MTT assay showing that ACCA treatment (250 μm) and GlaB treatment (5 μm) at indicated time points reduced growth of GL261 cells and that this effect was higher when cells were treated with both. Data are expressed as mean ± se, n = 3 (each in quadruplicate), *p < 0.05 vs control, #p < 0.05 vs GlaB+ACCA by One-way ANOVA followed by Student-Neuman-Keuls (SNK) post-test. b Cytofluorimetry study showing that the treatment of GL261 with ACCA (100 μm, 48 h) increases the percentage of apoptotic cells (n = 4, One-way ANOVA, SNK test, data are expressed as mean ± se, ***p < 0.001 vs control). c Cytofluorimetry study showing that the co-treatment of GL261 with GlaB and ACCA (5 μm and 100 μm respectively) for 48 h increases the percentage of cells featuring non-apoptotic/necrosis like death, measured with Propidium Iodide (PI) staining (n = 4, One-way ANOVA, SNK test, ***p < 0.001 vs control)