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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: 1H-NMR metabolomics reveals the Glabrescione B exacerbation of glycolytic metabolism beside the cell growth inhibitory effect in glioma

Fig. 1

Murine glioma GL261 cells are Hedgehog dependent tumor cells. a Data (mean ± se) are normalized to Gapdh mRNA expression and are expressed as Gli1 mRNA fold increase vs Gli1 mRNA expression levels on astrocytes. One-way ANOVA, **p < 0.01, n = 7. b Immunofluorescence images of Gli1 protein (RED) in Astrocytes, GL261 and GL261 + GlaB treated (5 μm, 48 h) cells. c Protein signal quantification in Astrocytes, GL261 and GL261 + GlaB treated (5 μm, 48 h) cells. Nuclei were visualized by Hoechst (BLU) scale bar: 20 μm. One-way ANOVA, **p < 0.01, n = 10. d MTT growth curves in untreated and GlaB treated cells (5 μm, 24 h, 48 h, 72 h) data are expressed as mean ± se, *p < 0.05, n = 3 (each in quadruplicate). e Apoptosis measured as the sum of cells positive for Annexin V and Annexin V/PI double positive cells in GL261 untreated or treated with GlaB 5 μm for 48 h, data are expressed as mean ± se, ***p < 0.001, n = 3 (each in duplicate)

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