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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling

Fig. 8

Small-molecule compounds inhibit tumor growth by targeting Wnt5b-mediated non-canonical WNT signaling and CK1α-mediated canonical WNT signaling in vitro and in vivo. a Chemical structure of LGK-974 and pyrvinium pamoate. Cell viability was determined in MDA-MB-231 and Bcap-37 cells following treatment with LGK-974 (b) and pyrvinium pamoate (c) for 72 h, mean ± SEM is shown (assays performed in quadruplicate). d LGK-974 and pyrvinium pamoate inhibited cell lines with a high expression of Wnt5b and a low expression of CK1α by foci formation assay. e Graphs represent LGK-974-treated or pyrvinium pamoate-treated Hs-578 T and Bcap-37 with or without Wnt5b (500 ng/ml), mean ± SD (n = 3 per treatment group), (n.s.: no significant, *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001). f Images of Bcap-37 cells bearing mice under 530 nm laser irradiation at day 5, 10, and 15 following treatment with LGK-974 (5 mg/kg) or pyrvinium pamoate (1 mg/kg). Tumor weight (g), Tumor volume (h), Tumor growth curve (i), and subcutaneous tumor (j) of Bcap-37 at the experimental endpoint. k Representative images of Ki-67 staining by IHC after 14 days of treatment

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