Fig. 3

miR-21a regulates the neurogliogenic decision of NPCs. (a, e) A schematic representation of the LOF (a) and GOF (e) approaches for miR-21a. (b, f) The expression levels of miR-21a and transcripts corresponding to β-tubulin and GFAP were determined by qPCR. (c, g) NPCs were transfected with either inhibitor control/miR-21a inhibitors (c) or mimics control/miR-21a mimics (g) with exosomes for 7 DIV in differentiation conditions. Representative images of Tuj1 (red), GFAP (red) and DAPI (blue) staining were shown. (d, h) Quantification of Tuj1⁺ and GFAP⁺ cells (as a percentage of total cells) in the culture. Data were represented as mean ± SD from three independent experiments. *, **, *** and **** denote p < 0.05, p < 0.01, p < 0.001 and p < 0.0001 in comparison to control, respectively. Scale bar 100 μm (c, g)