Fig. 1

EXOs promote neuronal differentiation of NPCs. (a) A schematic representation of the experimental approach. (b) The uptake of PKH26-labeled exosomes by NPCs was determined by immunocytochemical analysis. (c) NPCs were co-cultured with exosomes for 7 DIV in differentiation conditions. Representative images of Tuj1 (green), GFAP (red) and DAPI (blue) staining were shown. (d) Quantification of Tuj1⁺ and GFAP⁺ cells (as a percentage of total cells) in the culture. (e) The transcript expression of β-tubulin and GFAP post exosome treatment was determined by qPCR analysis. Data were represented as mean ± SD from three independent experiments. *, *** and **** denote p < 0.05, p < 0.001 and p < 0.0001, respectively. n.s. denotes no significance. Scale bar 20 μm (b) and 100 μm (c)