Fig. 5

The level of T cell-mediated damage on neurons was significantly reduced following an in vitro TIM-1 knockdown in co-cultured T cells and neurons. a Western Blot detection of the TIM-1 transfection efficiency. b The level of LDH expression assessed by an ELISA.**p < 0.01. c-d An Edu incorporation assay was used to examine the level of cellular proliferation. Representative images and quantification was represented by histogram. **p < 0.01; ***p < 0.001. e Light microscopy for the detection of neuronal cell morphology. f-i The detection of cytokines secreted, including TNF-α, MIP-1α, NO, and IL-6 in each group by ELISA. **p < 0.01