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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: The critical role of RasGRP4 in the growth of diffuse large B cell lymphoma

Fig. 3

Knockdown of RasGRP4 led to injured mitochondria and upregulated caspase-3 activity and oxidative stress in DLBCL. (a) Representative WB images of the cleaved caspase-3 expression in the NC and shRNA groups. (b) Quantification of the ratio of cleaved caspase-3/procaspase-3 (in a), which showed that the ratio was significantly higher in RasGRP4-knockdown groups than NC groups after analysis by ImageJ. (c) Representative histograms of mitochondrial membrane potential measured by flow cytometry with JC-1 probe. (d) Quantification of the red/green fluorescence ratio (in c), which was significantly lower when RasGRP4 was downregulated. The levels of oxidative stress markers ROS (e), MDA (f), and SOD (g) were determined using commercial assay kits. RasGRP4 knockdown significantly increased the oxidative stress in lymphoma cells. (h) RasGRP4 knockdown resulted in lymphoma cell injury. Intracellular LDH content was significantly higher in the knockdown groups than in the NC groups. N indicates the number of independent experiments. **P < 0.01, *P < 0.05

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