Fig. 2

Effective knockdown of RasGRP4 by lentiviral shRNA plasmids and result in decreased DLBCL cell proliferation. (a) Representative images of WB analysis, which show the different efficiencies of the three shRNAs in decreasing RasGRP4 expression in DLBCL. (b) Quantification of the densitometric assessment of protein bands (in a) using ImageJ software. The expression levels of RasGRP4 after transfection with shRNA-1, − 2, and − 3 were 72, 29, and 44%, respectively. (c) RasGRP4 mRNA levels were evaluated by qRT-PCR. N indicates the number of independent experiments. (d) Quantification of the cell number in the two groups (in A). The number of DLBCL cells was significantly lower in the RasGRP4-knockdown group compared with control. (e) Growth of RasGRP4-knockdown SUDHL-4 cells was significantly slower than the controls. (f) RasGRP4 knockdown disrupted the SUDHL-4 cell cycle. (g) Representative histograms of the apoptosis rate measured by flow cytometry in the RasGRP4-knockdown and control groups. (h) Quantification of apoptosis rate (in g), which showed that the proportion of cells in early and late apoptosis was significantly increased by RasGRP4 knockdown. N indicates the number of independent experiments. **P < 0.01