Fig. 6
From: Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells
Osteoblastic differentiation of Human Gingival Mesenchymal Stem Cells associates with induction of autophagy. HGMSCs were plated on sterile coverslips, let adhere and cultured for 1 to 21 days in control medium or in differentiation medium (Diff) supplemented or not with 1 μM resveratrol (RV). At the end, the coverslips were fixed and processed for immunofluorescence staining of the autophagy markers LC3 (marker of autophagosomes) and LAMP1 (marker of lysosomes). Fluorescence staining was quantified with the ImageJ software. Integrated fluorescence intensity of co-labeled area (yellow) was calculated and reported in histograms