Fig. 7

CB-839 treatment undermines estrogen-induced UEC growth in vivo. UEC xenograft was constructed in nude mice by subcutaneously injecting Ishikawa cells. a, b The mean fluorescence intensity (MFI) of Ki67 (a proliferation-related molecule) in UEC of control and CB-839-treated groups, was evaluated by flow cytometry. c The tumor volume was measured every 3 days. d, e Mice were sacrificed after 22 days and the weights of xenografts were recorded. f tumor tissues were separated digested, and then flow cytometry was used to reflect the MFI of Ki67 in cell suspension of UEC. g, h TEM was performed to analyze the autophagic degree, which was presented as APs and ALs per visual field. i Tumor volume was measured every 3 days. j Xenograft in mice. k Mice were sacrificed after 16 days and the weight of xenografts were measured. The data are expressed as the mean ± SD. *P < 0.05 or ***P < 0.001 and ns are regarded as of no statistical significance. E: estrogen-treated group, C: CB-839-treated group, E + C: estrogen plus CB-839-treated group