Fig. 6

CB-839 induces autophagy and inhibits proliferation of estrogen-sensitive and insensitive UECC. a Gln metabolism model diagram. b-g After stimulation with or without estrogen (10 nM), or estrogen (10 nM) plus CB-839 (1 μmol/L) for 48 h, cell viability (b, c), the expression of autophagy-related protein, LC3B, Beclin-1 and p62 (d, e), and the autophagic level (f, g) of Ishikawa and KLE cells were analyzed by the CCK-8, western blotting, and TEM analysis. Gray scale of protein expression was analyzed by image J software (f, g right). The data are expressed as the mean ± SD. *P < 0.05, or **P < 0.01 and ns means no statistical significance. E: estrogen-treated group, C: CB-839-treated group, E + C: estrogen plus CB-839-treated group