Fig. 3

Estrogen-induced GLS in UECC is dependent on c-MYC. a Bioinformatics analysis was applied to predict regulatory factors associated with GLS. b Gene-array heat map of WT mice and EAAE mice models treated with or without estrogen. c, d MYC (c-MYC, n-MYC and l-MYC) levels of WT mice (c) and EAAE mice (d) with or without estrogen treatment. e c-MYC, n-MYC and l-MYC levels of Ishikawa cells with or without estrogen treatment were detected by qRT-PCR. f Protein levels of MYC (c-MYC, n-MYC and l-MYC) in Ishikawa cells were tested by western blot. g After stimulation with estrogen (10 nM) and/or fulvestrant (250 nM) for 48 h, western blotting assay was used to evaluate the expression of GLS in Ishikawa cells. The data are expressed as the mean ± SD. **P < 0.01; *** P < 0.005; ****P < 0.001 and ns are regarded as of no statistical significance