Fig. 2From: Estrogen inhibits autophagy and promotes growth of endometrial cancer by promoting glutamine metabolismEstrogen stimulates Gln metabolism by up-regulating the expression of GLS. After treatment with estrogen (10 nM) and/or fulvestrant (250 nM) for 48 h, Ishikawa and KLE cells and supernatant were collected. a, b ELISA assay was used to test Gln in supernatant (a) and lysis solution (b). c, d Western blotting was carried out to evaluate the expression of GLS in protein. e, f Immunofluorescence of GLS (green) in Ishikawa and KLE was analyzed by laser confocal scanning microscope. The data are expressed as mean ± SD. **P < 0.01Back to article page