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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Novel interactions between ERα-36 and STAT3 mediate breast cancer cell migration

Fig. 6

STAT3 phosphotlyation is required for IL-6 induced MMP2 and MMP9 expression and breast cancer cell migration. a and b, MDA-MB-231 that were transfected with pCDNA3.1-YFP or pCDNA3.1-mSTAT-3, 48 h after transfection, cells were treated with and without IL-6 for 1 h; cell extracts were prepared and analyzed by Western blotting for STAT3 acetylation using anti-acetyl-STAT3 antibodies (a) or immunoprecipitated (IP) with anti-ERα-36 antibodies, and the immunocomplexes were analyzed for acetyl-STAT3 as described in A (b). IB, immunoblot. Blots were re-probed sequentially with anti-pSTAT3, anti-STAT3, anti-MMP2, anti-MMP9, anti-ERα-36, and/or anti-GAPDH antibodies. c and d, MDA-MB-231 cells were transfected with pCDNA3.1-YFP or pCDNA3.1-mSTAT3 in combination with either empty vector or pGL3-MMP2-luc (c) or pGL3-MMP9-luc (d) and analyzed for IL-6-induced luciferase activity. RLU, relative luciferase units. e, MDA-MB-231 cells were transfected with pCDNA3.1-YFP or pCDNA3.1-mSTAT3, quiesced and subjected to IL-6-induced MDA-MB-231 cell migration. (**, P<0.01, #, P>0.05) n = 3

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