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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Novel interactions between ERα-36 and STAT3 mediate breast cancer cell migration

Fig. 3

ERα and STAT3 mediates IL-6 induced MMP2 and MMP9 promoter activity a, Schematic of the − 904 MMP2 promoter and − 4136 MMP9 promoter, containing GAS element was used for luciferase reporter assay. b and c, After MDA-MB-231 cells were treated with IL-6 for the indicated time periods. Then the luciferase reporter assays was used to test the transcriptional activity of MMP2 (b) and MMP9 (c). d and e, After MDA-MB-231 cells were transfected with ERα-36 or STAT3 for 24 h. Then the luciferase reporter assays was used to test the tanscriptional activity of MMP2 (d) and MMP9 (e). f, 24 h post-transfected with ERα-36 or STAT3, total proteins were extracted and the protein level of MMP2 and MMP9 were detected by Western blotting analysis. GAPDH was used as a loading control. g and h, MDA-MB-231 cells were transfected with ERα-36 or STAT3 for 24 h, total RNA was isolated and the expression of MMP2 and MMP9 were examined by qPCR. GAPDH was used to serve as a loading control. (**, P<0.01, #, P>0.05) n = 3

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