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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Novel interactions between ERα-36 and STAT3 mediate breast cancer cell migration

Fig. 2

STAT3 mediates IL-6 induced MMP2 and MMP9 expression and breast cancer cell migration. a and b, MDA-MB-231 cells were either transduced with Ad-GFP or Ad-GFP-STAT3(a) or transfected with scrambled (Scr) or STAT3 siRNA (100 nM) (b), After 48 h, cells were treated with or without IL-6 for 2 h, and cell extracts were prepared. And analyzed by Western blotting for MMP2 and MMP9 levels using its specific antibodies. The blot was re-probed with anti-GAPDH antibodies for normalization. c and d, all the conditions were the same as in a or b except that quiescent MDA-MB-231 cells were subjected to IL-6-induced migration assay (C and D). (**, P<0.01, #, P>0.05) n = 3

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