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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Novel interactions between ERα-36 and STAT3 mediate breast cancer cell migration

Fig. 1

IL-6 induces MMP2 and MMP9 expression in breast cancer cell migration. a, b, c and d, MCF-7 and MDA-MB-231 cells were treated with and without IL-6 for the indicated time periods, and either total cellular RNA was isolated and analyzed for STAT3, MMP2 and MMP9 mRNA levels by qPCR (a and b) or cell extracts were prepared and analyzed for STAT3 phosphotlyation, MMP2 and MMP9 levels by Western blotting using its specific antibodies (c, d and e, f). The blot was reprobed for GAPDH for normalization. G, MDA-MB-231 cells were transfected with scrambled (Si-NC) or MMP2 or MMP9 siRNA (100 nM), after 48 h, then cells were treated with or without IL-6 for 2 h, Cell migration was measured by modified Boyden chamber method. (**, P<0.01, #, P>0.05) n = 3

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