Fig. 4

High-salt intake enhances NFAT5 expression and nuclear translocation in ECs. a Quantification of mRNA levels of NFAT5 in TA and AA of ApoE−/− mice fed with a normal or high-salt diet. b En face immunofluorescent staining of NFAT5 in ECs of TA and AA of ApoE−/− mice fed with a normal or high-salt diet. Circles mark the cells with nuclear NFAT5 expression. c, d mRNA and protein levels of NFAT5 in HUVECs exposed to iso- and hyper-osmotic media, evaluated by RT-qPCR and western blotting with β-actin as the internal control. All data were presented as mean ± SEM, N ≥ 3. *p < 0.05