Fig. 2From: Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81 and Ser213CAPE treatment suppressed protein level of AR and PSA but not mRNA of AR. Protein expression level of AR, phospho-AR Ser308 in LNCaP 104-S cells (a) and 104-R1 (b) cells as well as PSA in in LNCaP 104-S cells (c) and LNCaP 104-R1 cells (d) treated with DHT and CAPE for 48 h was determined by Western blotting. The β-actin was used as loading control. The mRNA expression level of AR in LNCaP 104-S cells (e) and LNCaP 104-R1 cells (f) treated with indicated concentration of DHT and CAPE for 48 h was analyzed by qRT-PCR. GAPDH was used as loading controlBack to article page