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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: WDR76 degrades RAS and suppresses cancer stem cell activation in colorectal cancer

Fig. 6

WDR76 regulates CSC activation in CRC spheroids harboring oncogenic KRAS mutation. a-d Five-day spheroid cultures of D-MT cells stably expressing GFP-Control or GFP-WDR76 were analyzed. a Number and size of spheroids were quantified using Image J. *** p < 0.001. * p < 0.05, ** p < 0.01. b Extracts were immunoblotted using the indicated antibodies. c Relative mRNA levels of the indicated genes were quantified by RT-qPCR. * p < 0.05, ** p < 0.01, and *** p < 0.001. d Immunocytochemistry was performed using the indicated antibodies and counterstaining with DAPI. Scale bars represent 20 μm. e-h Five-day spheroid cultures of D-MT cells stably expressing shControl-GFP or shWDR76-GFP were analyzed. e Number and size of spheroids were quantified using Image J. * p < 0.05, ** p < 0.01. f Extracts were immunoblotted using the indicated antibodies. g Relative mRNA levels of the indicated genes were quantified by RT-qPCR. * p < 0.05, ** p < 0.01, and *** p < 0.001. h Immunocytochemistry was performed using the indicated antibodies and counterstaining with DAPI. Scale bars represent 20 μm

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