Fig. 3

Loss of Wdr76 activates the Wnt/β-catenin pathway in CRC. a Western blot of D-WT and D-MT cells after treatment with AS703026 and LY294002. b Relative mRNA levels of CSC markers in D-WT and D-MT cells treated with AS703026 and LY294002. c Immunohistochemical staining of Ras and β-catenin in intestinal sections of 15-week-old Apc^Min/+ and Apc^Min/+; K-Ras^G12DLA2 mice. Boxes indicate the enlarged areas. Scale bars represent 100 μm (left panel) and 20 μm (right panel). d Western blots of tumors extracts from 15-week-old Wdr76^+/+; Apc^Min/+and Wdr76^−/−; Apc^Min/+ mice incubated with the indicated antibodies. e Confocal immunofluorescence of Wdr76 and β-catenin in small-intestine sections of 15-week-old Wdr76^+/+; Apc^Min/+ and Wdr76^−/−; Apc^Min/+ mice. Boxes indicate the enlarged areas. Tumors are indicated by dotted lines. T: Tumor. Scale bars represent 100 μm (left panel) and 20 μm (right panel). f Confocal immunofluorescence of Wdr76 and β-catenin in small-intestine sections of 15-week-old Wdr76^+/+ and Wdr76^−/− mice. Boxes indicate the enlarged areas. Intestinal crypts are indicated by dotted lines. Scale bars represent 100 μm (left panel) and 20 μm (right panel). g Schema depicting the effects of Wdr76 deletion in CRC harboring Apc mutation