Fig. 1
From: WDR76 degrades RAS and suppresses cancer stem cell activation in colorectal cancer
Loss of Wdr76 increases Ras protein level and crypt length in the murine small intestine. a Confocal immunofluorescence of Wdr76 and Lgr5-GFP in Lgr5-EGFP mouse intestinal sections: Wdr76 (red), Lgr5-GFP (green), and DAPI (blue). b-e Analysis of small intestinal sections of 15-week-old Wdr76+/+ and Wdr76−/− mice b H&E stained sections of small intestine from Wdr76+/+ and Wdr76−/− mice and quantification of the length of small intestinal crypts. c Confocal immunofluorescence and quantification of PCNA in small-intestine sections of Wdr76+/+ and Wdr76−/− mice. d Confocal immunofluorescence of Wdr76 (red) and Ras (red) in intestinal sections of Wdr76+/+ and Wdr76−/− mice. e Confocal immunofluorescence of Ki67 and Ras in small-intestine sections of Wdr76+/+ and Wdr76−/− mice with quantifications of Ki67+ cells in crypts and Ras expression levels in Ki67+ crypt cells. a-e Boxes indicate the enlarged areas. Crypts are indicated by dotted lines. Scale bars represent 20 μm. All measurements or counts are based on at least 10 crypts per 5 fields of view. *** p < 0.001. f Schema depicting the effects of Wdr76 deletion (Wdr76 KO) in normal small intestine