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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Protein phosphatase 2A modulates podocyte maturation and glomerular functional integrity in mice

Fig. 6

Cell spreading and cytoskeleton rearrangement in podocyte from Pod-PP2A-KO mice. a Representative image of podocytes seeded on plates coated with fibronectin from Pod-PP2A-KO mice, monitored by live cell imaging for 30, 60 and 120 min. A modest but significant increase in cell spreading was observed in the isolated podocytes seeded on plates coated with fibronectin from Pod-PP2A–KO mice when compared with control. b Quantification of cell area was performed in each experiment. n = 3 experiments. c Primary podocytes were stained with a-tubulin and FITC labeled phalloidin to visualize the microtubule and F-actin cytoskeleton in physiological state, and no significant differences was observed. d Representative image of actin fiber in the primary podocyte after incubation with cytochalasin D for 30 min. The expression of actin fiber was significant down-regulated in primary podocytes after cytochalasin D treatment from Pod-PP2A-KO mice than that of control

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