Fig. 1

The expression of PP2A in human and ADM nephropathy animal models and Generation of podocyte-specific pp2a knock out mice. a Double immunofluorescence demonstrates PP2A expression in the podocyte of normal human kidney. Scale bars: 50 μm. b The expression of PP2A in the podocytes from ADM nephropathy model in the BALB/c mice (n = 5 per time point). Scale bars: 50 μm. c The results of Western blotting indicate the PP2A expression in purified control (Ctrl) podocytes and lack of PP2A expression in podocytes harvest from the PP2A specific-knock out mice. d Identification of pp2a and Podocin-Cre DNA level by tail genotyping (4 week). e Primary podocytes isolated from Pod-PP2A-KO mice were plated on collagen type I–coated glass coverslips and then stained for WT1 (Red) and PP2A (Green). Compared with the control, the expression of PP2A was also down-regulated in primary podocytes from Pod-PP2A-KO mice. Scale bars: 25 μm