Fig. 6
From: A novel population of extracellular vesicles smaller than exosomes promotes cell proliferation
Exosome depleted FBS promotes cell proliferation but not exosome production. a Western blot of the P100 and P200 fractions isolated from the fresh 10% FBS and 10% ED-FBS media for detecting Hsp70. b The number of particles in the P200 fractions in different media: SF, 10% ED-FBS, 10% FBS. c The P100 fractions isolated from HEK293 CM under different culture conditions: SF, 10% FBS, 10% ED-FBS. The levels of Hsp70 are shown. d The cell number of HEK293 cells cultured in different media: SF, 10% FBS, 10% ED-FBS. e, f The fresh SF media, SF + liposomes, SF + P100 and SF + P200 fractions prepared from 10% ED-FBS media were supplied to the same number of THP-1 cells (dark gray). SN0, SNΔ1, SNΔ2 and SNΔ2 + P100 fractions prepared from the 10% ED-FBS media were supplied to THP-1 cells (light gray). (e) The final cell numbers of THP-1 cell culture, and (f) the number of exosomes normalized by the final cell numbers. Blue arrows indicate marked effects by the P200 addition and red arrowheads indicate insignificant changes by the P100 addition. Experiments were repeated three or six times, each including six technical repeats. The results are displayed as mean ± S.E.M. *Significant difference analyzed by t-test (*p < 0.05,**p < 0.01,***p < 0.001). ns = not significant. Full original blots are presented in Additional file 8: Figure S8f, g