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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: A novel population of extracellular vesicles smaller than exosomes promotes cell proliferation

Fig. 5

Depletion of P100 fraction reduces exosome production while depletion of P200 fraction reduces cell proliferation. a A scheme to show experimental procedures. SN0, SNΔ1, SNΔ2 and SNΔ2 + P100 fractions prepared from HEK293 CM and 10% FBS culture media were supplied to either THP-1 or HEK293 cell culture. b-d When HEK293 cells were treated with the fractions prepared from HEK293 CM cultured in SF. The bar graphs show (b) the final cell numbers, (c) the number of exosomes per cell, and (d) the net production of exosomes (EV48h – EV0h). e, f HEK293 cells were treated with the fractions prepared from HEK293 CM cultured in 10% FBS. The bar graphs show (e) the final cell number and (f) the number of exosomes per final cell number for THP-1 (dark green) and HEK293 cells (light green). g, h HEK293 cells were treated with the fractions prepared from HEK293 CM cultured in 10% FBS. The bar graphs show (g) the final cell numbers and (h) exosome number per cell of THP-1 (dark blue) and HEK293 cells (light blue). Red and blue arrows indicate insignificant effects of the P100 and the significant effects of the P200 addition, respectively. Experiments were repeated three or six times, each including six technical repeats. The results are displayed as mean ± S.E.M. *Significant difference analyzed by t-test (*p < 0.05,**p < 0.01,***p < 0.001). + p < 0.1 and ns = not significant

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