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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: A novel population of extracellular vesicles smaller than exosomes promotes cell proliferation

Fig. 4

P100 fraction promotes exosome secretion while P200 fraction promotes cell proliferation. a A scheme to show experimental procedure. The fresh SF media, SF + P100 and SF + P200 fractions were supplied to the same number of THP-1 cells and HEK293 cells. After 48 h culture, the number of exosomes and cells were counted. b-d P100 and P200 fractions were prepared from HEK293 CM (SF), and were added to SF media. Liposomes were added as a negative control. The bar graphs show (b) the final cell numbers, (c) the number of exosomes per cell, and (d) the net production of exosomes per cell (EV48h – EV0h) of HEK293 cell culture after each addition. (e, f) HEK293 cells were treated with the fractions prepared from 10% FBS. The bar graphs show (e) final cell numbers of THP-1 (dark green) and HEK293 cells (light green), and (f) the number of exosome normalized to the final cell numbers. g, h THP-1 or HEK293 cells were treated with the fractions prepared from HEK293 CM cultured in 10% FBS. The bar graphs show (g) the final cell numbers for THP-1 (dark blue) and HEK293 cells (light blue), and (h) the number of exosomes normalized to the final cell numbers. Red and blue arrows indicate marked effects by the P100 and the P200 fractions, respectively. Experiments were repeated three or six times, each including six technical repeats. The results are displayed as mean ± S.E.M. *Significant difference analyzed by t-test (*p < 0.05,**p < 0.01,***p < 0.001). + p < 0.1 and ns = not significant. SF = serum-free

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