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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: A novel population of extracellular vesicles smaller than exosomes promotes cell proliferation

Fig. 3

P100 and P200 fractions contain the different levels of exosome markers. a-d Sub-fractions were prepared from CM of HEK293 cells grown in SF condition for 72 h by ultracentrifuation and used for western blots to check the following exosome markers: (a) Hsp70, (b) TSG101, (c) CD63, and (d) CD81 (SBI, rabbit). Red arrow indicates the expected target protein size in each panel. e Western blot of the P100 and P200 fractions from CM of HEK293 cultured in 10% FBS media using anti-CD81 antibody. Red arrow indicates the CD81 protein. f, g Representative TEM images of the vesicles in (f) P100 and (g) P200 from the HEK293 CM labeled with 10 nm gold particles resulted from staining with an anti-CD63 antibody and anti-rabbit gold conjugated antibody. Red arrows indicate the CD63 protein. Original full blots are presented in Additional file 8: Figure S8a-e

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