Fig. 2

LAT3 is required for PI3K/Akt stimulated leucine uptake. a LAT3 expression levels were examined by western blot in shControl and shLAT3 of LNCaP cells. All western blot images are representative of three independent experiments. b leucine uptake was examined in RPMI supplemented with dialyzed FBS, serum-free media, MK2206 or BCH, in both shControl and shLAT3 of LNCaP cells. Two-way ANOVA was performed. Data are the mean ± SEM, n = 3