Fig. 4
From: Connecting lysosomes and mitochondria – a novel role for lipid metabolism in cancer cell death
Alterations in mitochondrial function due to lysosomal stress. HUH7 cells were treated as indicated (24 h). DIOC6 (a) or JC1 (b) fluorescence were quantified by flow cytometry. c Enzyme abundance was quantified by flow cytometry. d Relative mRNA expression levels of ACADVL*, ACADM# and HADHA† were assessed by qPCR. a-d Bars are the SEM of three independent experiments. p*#† < 0.05 (One-way ANOVA, Dunnett post test) (e) Cells were fixed and stained for ACADVL, ACADM and HADHA, respectively, (green) and nuclei (blue) and analyzed by confocal microscopy. Scale bar 7.5 μm. Representative images out of three independent experiments are shown