Fig. 3
From: Connecting lysosomes and mitochondria – a novel role for lipid metabolism in cancer cell death
Mitochondrial structure is changed upon V-ATPase inhibition. HUH7 cells were treated as indicated (24 h). a, c, d Representative images out of three independent experiments are shown. a Cells were fixed and stained for the mitochondrial marker Hsp60 (magenta), lysosomal marker Lamp3 (cyan) and nuclei (yellow) and analyzed by confocal microscopy. Scale bar 25 μm. b Mitochondria were stained with MitoTracker™ Green FM and mitochondrial mass was assessed by flow cytometry immediately. c Protein level was detected by WB. Total protein served as loading control. Quantification of Drp1 phosphorylation (bar graph). d Mitochondrial morphology was analyzed by TEM. Scale bar 500 nm. e Relative change in mitochondrial area was assessed using ImageJ. At least fifty mitochondria from TEM images (d) have been analyzed. f Feret diameter of at least fifty mitochondria from TEM images (d) was calculated using ImageJ. Bars are the mean + SEM of three independent experiments. p* < 0.05 (One-way ANOVA, Dunnett post test)