Fig. 1
From: Connecting lysosomes and mitochondria – a novel role for lipid metabolism in cancer cell death
V-ATPase inhibition influences lipid profile. Cells were treated as indicated (24 h). Lipids from whole cells (HUH7, HepG2 and Hep3B) (a), lysosomes (HUH7) (b) or mitochondria (HUH7) (c) were isolated and TAG composition was analyzed by UPLC-MS/MS. Heatmaps display percentage increase (red) and decrease (blue) of respective TAG species compared to DMSO control. d Lipids from whole cells (HUH7) were isolated and cholesteryl ester composition was analyzed by mass spectrometry (student t-test). e, f Cells were loaded with Bodipy 493/503 to stain lipid droplets (LD). e LD size and localization was analyzed by confocal microscopy. Scale bar 10 μm. Representative images out of three independent experiments are shown. Bars are the mean + SEM of three independent experiments. f LD content was quantified by flow cytometry. p* < 0.05 (One-way ANOVA, Dunnett post test)