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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Inhibition of cyclin E1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression

Fig. 4

Inhibition of CCNE1 induced HCC apoptosis by targeting Mcl-1. a The expression of the target proteins in Huh7 cells treated with 50 nM Din at the specified time points. b The expression of Bim and Mcl-1 in Huh7 cells transfected with the CCNE1 or control plasmid and treated with 8 μM regorafenib. c The expression of Bim and Mcl-1 in Huh7 cells transfected with CCNE1 or control siRNA and treated with 8 μM regorafenib. d The expression of Mcl-1 in Huh7 cells treated with 50 nM Din in combination with 8 μM regorafenib or 5 μM sorafenib. e Hoechst 33258 staining for apoptosis of Huh7 cells transfected with the control or Mcl-1 plasmid and treated with 50 nM Din in combination with 8 μM regorafenib. f The expression of cleaved caspase-3 and Mcl-1 in Huh7 cells transfected with the control or Mcl-1 plasmid and treated with 50 nM Din in combination with 8 μM regorafenib. The western blots were repeated for 3 times, and representative data were shown. N = 3 for e. *, P < 0.05, **, P < 0.05

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