Fig. 3

Inhibition of CCNE1 sensitized hepatocellular carcinoma cells to regorafenib and sorafenib induced apoptosis. a The cell viability of Huh7 and HepG2 cells treated with 50 nM Din. b The cell cycle of Huh7 cells treated with 50 nM Din. c Hoechst 33258 staining for apoptosis of Huh7 and HepG2 cells treated with 50 nM Din at the indicated time points. d Annexin V/PI staining for apoptosis of Huh7 and HepG2 cells treated with 50 nM Din at the indicated time points. e Western blot of cyclin E1, and cleaved caspase 3/8 in Huh7 cells treated with 50 nM Din at indicated the time points. f Hoechst 33258 staining for apoptosis of Huh7 and HepG2 cells treated with 50 nM Din in combination with 8 μM regorafenib or 5 μM sorafenib. g Hoechst 33258 staining for apoptosis of Huh7 and HepG2 cells treated with 100 nM FLA in combination with 8 μM regorafenib or 5 μM sorafenib. The western blots and flow cytometry were repeated for 3 times, and representative data were shown. N = 3 for a, c, d, f, g. *, P < 0.05, **, P < 0.05, ***, P < 0.001